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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Microcentrifuge tubes Micropipettor and tips Rotator Sonicator Spectrometer (Qubit Systems) Test tubes, conical, 50- and 250-mL Thermal cycler Tubes (microcentrifuge) Vortex mixer Water baths or heating
Replication timing by density transfer
on the frozen azide/EDTA and immediately add the cell sample. Vortex or shake the tube vigorously to chill the sample and break up the frozen EDTA. Spin down the cells in a chilled centrifuge, wash with 1 ml cold water per sample in Eppendorf tubes and freeze
The ribonuclease protection assay (RPA)
in RNase-free water at -70°C. Add the desired amount of target RNA (generally 1-20 µg) to 1.5 ml Eppendorf tubes and include a tube that contains yeast tRNA as a background control. In general, 20-24 total sample tubes are an easily manageable number
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