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文献和实验Proteasome Preparation from Human Brain
) Read OD @ 595nm. d) Repeat assay using standards i.e. Chicken gamma globulin at known concentrations. N.B.: Fractions 1 to 11 - 10ul sample + 40ul water. Fraction 12 onwards 50ul undiluted sample.
Two Dimensional Gel Electrophoretic Analysis for the Human Plasma Proteome
from the vertical glass plates before loading polyacrylamide gel solution. Reference N. Leigh Anderson, Norman G. Anderson. The Human Plasma Proteome. History, character, and diagnostic prospects. Molecular & Cellular Proteomics 2002;1.11:845-67.
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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