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上海玉博生物科技有限公司
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文献和实验相关专题 (一)材料及试剂 1.培养基 RPMI-1640 或TC199培养液 2.PHA 同形态学方法 3.小牛血清 4.3H-TdR 选用比活性为2Mci~10Mci/mg分子 的制品,将1 Mci/ml溶液以生理盐水稀释成100µci/ml,于4℃保存备用。临用时再用培养液稀释成10µci/ml溶液。 5.3%冰醋酸 6.浓甲醛 7.30%H2O2液 8.闪烁液 PPO(2,5二苯基噁唑
H. Genomic DNA isolation from blood
samples typically were obtained as 1 ml of whole blood stored in EDTA vacutainer tubes frozen at -70deg C. 2. Thaw the frozen samples, and to each 1 ml sample, add 0.8 ml 1X SSC buffer, and mix. Centrifuge for 1 minute at 12,000 rpm
Studies of Respiration in H. pylori
is achieved using a Clark-type oxygen electrode linked to a chart recorder. The volume of cell suspension required is of the order of 1 mL (0.5–1 mg cell protein mL) and the method is sufficiently sensitive to detect the consumption of a few nmol oxygen per mL
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