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文献和实验-expression analysis (8 -10 ). An outline of gene cloning and expression profiling by anchored-PCR with vector primers is illustrated in Fig. 1B . In this chapter, we focus on how to use a gene library and anchored-PCR for cloning unknown gene sequences
Preparation and Use of Subtractive cDNA Hybridization Probes for cDNA Cloning
Molecular cloning is the process of inserting foreign fragments of DNA into a plasmid or bacteriophage vector that is capable of autonomous replication in a suitable host cell. The resulting recombinant DNA molecules
Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors
cloning vehicle, and, finally, cells must be transformed with the chimeric vector/cDNA molecules. Recent improvement in cDNA synthesis protocols (1 ) and in the quality of commercially available reverse transcriptase enables double-stranded cDNA
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