SBI特约代理商|CD513B-1 pCDH-CMV-MCS

Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers

-expression analysis (8 -10 ). An outline of gene cloning and expression profiling by anchored-PCR with vector primers is illustrated in Fig. 1B . In this chapter, we focus on how to use a gene library and anchored-PCR for cloning unknown gene sequences

Preparation and Use of Subtractive cDNA Hybridization Probes for cDNA Cloning

Molecular cloning is the process of inserting foreign fragments of DNA into a plasmid or bacteriophage vector that is capable of autonomous replication in a suitable host cell. The resulting recombinant DNA molecules

Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors

cloning vehicle, and, finally, cells must be transformed with the chimeric vector/cDNA molecules. Recent improvement in cDNA synthesis protocols (1 ) and in the quality of commercially available reverse transcriptase enables double-stranded cDNA