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文献和实验%1mg/9g细胞Rho1D4 IAC+SECOR131-2 G蛋白偶联受体可诱导的HEK293S细胞系(哺乳动物)90%2.9mg/10g细胞Rho1D4 IAC, +centrifugationhOR17-4 G蛋白偶联受体可诱导的HEK293S细胞系(哺乳动物)90%7.5mg/2.5L, 培养物Rho1D4 IAC+SEChOR17-4 G蛋白偶联受体无细胞小麦胚芽提取物70%*0.3mg/mL, 反应溶液*Rho1D4 IAC+SECCD81 四次跨膜
Characterization of Histaminergic Receptors
( protocol 2 ) Basic Protocol 4: Measurement of [3H]histamine Binding to Cloned Human H4 Receptors in Membranes Materials Cell lines (e.g., human HEK‐293, ATCC# CRL‐1573; or SK‐N‐MC, ATCC# HTB‐10) transfected (Liu et al
Intracellular Cytokine Staining Protocol
stimulation period for induction of a given cytokine is variable and has to be determined. For example, the best time for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 needs at least 24 hours stimulation
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