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文献和实验Functional Assays on High‐Content Protein Microarrays
, K., and Tabunoki, H. 2009. Protein microarray analysis identifies human cellular prion protein interactors. Neuropathol. Appl. Neurobiol. 35:16‐35. Schweitzer, B., Predki
Strategies to Optimize Protein Expression in E. coli
., and Manjunath, P. 2009b. Recombinant expression and affinity purification of a novel epididymal human sperm‐binding protein, BSPH1. Mol. Hum. Reprod. 15:105‐114. Leichert, L
An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage
and purifi ed YFP-SUMO-1 and CFP-RanGAP1 as model substrates that are covalently coupled in the presence of recombinant SUMO E1 and E2 enzymes and ATP. Reactions of 25 μl volume, set up in 384-wells plates, give suffi cient signal for analysis. Consequently
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