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文献和实验Preparing Colloidal Gold for Electron Microscopy
2 ) for 30 min at 4°C. At the correct speed the gold particles will settle to the bottom of the tube as a loose pellet. Remove the supernatant without disturbing the pellet and re-suspend the loose part of the pellet in PBS containing 0.2% BSA.
Preparing Colloidal Gold for Electron Microscopy
centrifugation step down a gradient will remove any gold particles of the wrong size. This is done on a 10-30% continuous glycerol gradient at 4°C. Layer 1-2 ml of protein A gold onto the top of the gradient, spin in an SW40 rotor at the appropriate speed
When the agar solution has cooled to approximately 60o C quickly fill each tube with it, resuspend the samples and spin them at full speed for 30 secs - 1 min. (Maximum of 4 samples at a time or the agar will set before the sample can be spun
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