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文献和实验Preparing Colloidal Gold for Electron Microscopy
Preparation of Colloidal Gold Conjugates. Colloidal gold has been used for centuries in the preparation of stained glass for windows and fine glassware. In recent years, colloidal
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
3 tube with 10ul of 10mM Tris-pH=8, 1mM EDTA, and combine with label. (Volume = 67ul) Add the 0.1 ul of 1mg/ml C0T-1 DNA (Volume = 67ul) Genisphere recommends 20 ug of Linear Polyacrylamide (LPA) (2ul of 10ug/ul) for carrier. (Volume = 69ul)
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
dish (can store for a year). Coat with polyamino acid. Coat coverslips in bulk in 10-15ml 1mg/ml PLL (or 500ug/ml polyornithine), rocking or rotating for a minimum of 30 minutes in a 10 or 15cm tissue culture dish. Save the polyamino acid
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