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- 文献和实验
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上海玉博生物科技有限公司
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文献和实验.16 112.05 Dynabeads M-280 Streptavidin Supplied at 6.7x108 beads per ml (10mg/ml) in PBS, pH7.4 with 0.1% BSA and 0.02% sodium azide. Isolation of any biotinylated target. Applications include DNA, RNA and protein isolation
In Situ Hybridization Using Digoxigenin Labeled Probes
on two 5ml pipettes in a tray lined with moist paper towels. Apply 100µl of a 1:500 dilution of Anti Digoxigenin-POD in 1X PBS, 5mMEDTA. Distribute the solution by placing a 22 x 40 mm coverslip on the slide. 5) Cover the tray with plastic wrap
Bromodeoxyuridine Staining of Mammary Tissue
Triton X-100 100mL PBS 40 mL DAB Solution DAB Stock - 3'-3' diaminobenzadine, 2.5 mg/mL in 0.1M PO4, pH7.6. Unactivated DAB - 1 mL DAB + 19 mL 0.1M PO4 +450 mL 1% aq. NiCl2, pH7.6. Activated DAB - 10 mL unactivated DAB + 23 mL 30% H2O2.
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