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上海玉博生物科技有限公司
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文献和实验High Throughput Analysis of Nuclear ReceptorCofactor Interactions
. One of the most useful assays for studying direct protein—protein interactions is the glutathione-S -transferase “pulldown” assay. We have developed a high-throughput version of this assay that utilizes a filter microplate to allow parallel processing of many samples
Herein, we describe a flow cytometric assay, which allows us to measure the activity of six small GTPases simultaneously. GST-tagged small GTPases are bound to six glutathione bead sets each set having a different intensity of red fluorescence at a fixed wavelength
as given below: Low salt wash buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH8.0, 150 mM NaCl); High salt wash buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH8.0, 500 mM NaCl); LiCl buffer (0.25 M LiCl, 1% NP-40, 1% SDC
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