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上海玉博生物科技有限公司
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文献和实验Cloning of Taq polymerase-amplified PCR products
out the gel slice containing the PCR product and melt it at 65°C in 2 volumes of 6 M NaI. 4) Add 1.5 volumes Binding Buffer (provided in the S.N.A.P. ™ MiniPrep Kit). 5) Load solution (no more than 1 ml at a time) from Step 3 onto a S
miRNA microarrays(McManus Lab)(芯片)
Ambion 3x hybridization buffer (Cat # 1567 mirVana™ miRNA Bioarray Essentials Kit) 22x25 mm lifter sliPS (Erie 22x25I-M-5226) a) Heat 3x hyb buffer at 65℃ and vortex to resuspend b) Add 5 to 10 100 μL drops of 5x SSC
Denaturing Agarose Gel Electrophoresis of RNA
. We generally load 1 µg and 2.5 µg samples on 1% agarose gels in TBE (89 mM Tris-HCl pH 7.8, 89 mM borate, 2 mM EDTA) with 0.5 µg/ml ethidium bromide added to the gel. Add 10X native agarose gel loading buffer (15% ficoll, 0.25
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