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上海玉博生物科技有限公司
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文献和实验flasks Dulbecco's phosphate buffered saline, pH 7.4 (DPBS; Invitrogen, cat. no. 25200‐056): pH 7.4 0.05% trypsin‐EDTA
Native Chromatin Preparation and Illumina/Solexa Library Construction
, buffers (including buffer EB), and collection tubes. MNase digestion buffer for ChIP MNase stop buffer for ChIP NaCl NaOAc (sodium acetate) (3 M, pH 5.3) Phenol:chloroform (1:1) Phosphate-buffered saline (PBS) (1X
Western Blotting with Biotinylated Antibodies
SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). 2.Aspirate excess PBS. 3.Add 1ml boiling lysis buffer
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