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文献和实验chemistry. The first is pyroglutamate aminopeptidase, a thiol exoprotease that cleaves N-terminal pyroglutamyl residues (pyrrolidone carboxylic acid) from peptides and proteins (6 –10 ). N-terminal glutamine residues can readily cyclize to the pyroglutamyl
[资源] 申请上传 Current Protocols In Protein Science （60M） 见公共邮箱
Determination of Protein Concentration Unit 3.2 Quantitative Amino Acid Analysis Unit 3.3 In Vitro Radiolabeling of Peptides and Proteins Unit 3.4 Assays for Total Protein Unit 3.5 Kinetic Assay Methods Unit 3.6 Biotinylation of Proteins in Solution
DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
were loaded to (12 ¡Á 17 ¡Á 0.04 cm) 0.5X MDE (BMA, Rockland, ME, USA) gel containing 10% glycerol. Electrophoresis was carried out using 1X TBE (0.098 M Tris-Hcl, 0.098M boric acid, 10Mm EDTA) at room temperature with the power fixed at 200v for 5-6 h
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