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上海玉博生物科技有限公司
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文献和实验Citric Acid,3Na,2H2 O (柠檬酸钠) 250g 70 Amresco分装 B1066 Colchicine 秋水仙碱(素) 250mg 230 Sigma分装 B1067 Collagen I(I型胶原酶)
blood into 1,5 eppendorf tubes with ACD (acid-citrate-dextrose; Sigma C-3821) = 1 vol of ACD : 3 vol of blood : 6. 6 vol of NaN 3 0,05%-PBS Cetrifuge at 216-220g , 5-7min , 10ѓC Take & save at 4ѓC PRP (platelet-rich plasma )
Protocol for evaluating vascular permeability in wildtype
1) Female Tiel-PR Tg mice and wildtype control litter-mates aged 6-9 wks were ovariectimized and allowed to recover for 7-10 days. Making estrogen and progesterone stock solutions: For estrogen (17 beta-estradiol from Sigma), weigh out 10
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