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上海玉博生物科技有限公司
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
for bacterial cells #P-8849 from Sigma) and strong denaturant as 6M Guanidine-HCl (GuHCl) or 6 to 8M Urea Optional additives to the lysis buffer a) 1mM PMSF or protease inhibitor cocktail 1:200 (cocktail for bacterial cells #P-8849 from Sigma) b
Studies of the Ubiquitin Proteasome System
” (unlabeled) ubiquitin (Sigma) for immunoblot detection 4× nonreducing SDS‐PAGE sample buffer (see recipe ) 4× reducing SDS‐PAGE sample buffer (see recipe
B1134 Hepes(N- 2 -羟乙基哌嗪- N'-2-乙磺酸) 25g/50g/100g 100/180/300 Amresco分装 B1135 L-Histidine (L-组氨酸) 5g/10g 70/120 Sigma分装 B1136
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