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文献和实验(this sometimes happens) 10) add fungizone, antibiotics (pen and strep) and FBS to one label complete MEM 11) just add media to other and label incomplete MEM DMEM for PtK1s 1) rinse cylinder well with ddH2 O 2) add 600 mls ddH2 O 3) add 1 bottle
, antibiotics (pen and strep) and FBS to one label complete MEM 11) just add media to other and label incomplete MEM DMEM for PtK1s 1) rinse cylinder well with ddH2 O 2) add 600 mls ddH2 O 3) add 1 bottle DMEM 4) add 3.7g Sodium
美国密歇根大学的3T3-L1细胞分化的PROTOCOL(非常详细)3T3-L1 Differentiation ProtocolMATERIALSDulbecco's Modified Eagles Medium (DMEM; GibcoBRL-Cat# 11965-084: high glucose, with L-glutamine, with pyroxidine HCl, without sodium pyruvate)Calf Serum (GibcoBRL-Cat#16170
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