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Live imaging with Drosophila tissue culture cells

) (see note 3): Culture dishes or slides with coverslip bottom are used. Some providers produce round culture dishes (e.g. Willco Wells B.V.®) or 8-well or single well culture slides (e.g. Nunc™ or ibidi®, see figure 2); To follow

Transgenic Animals（图）

is used for moving the injection capillary.The actual holding of the embryo is carried out using CellTram Air.Two possibilities are available for injecting DNA: using the CellTram Air as a simple injection device or using the FemtoJet™ for reproducible

Cloning of Taq polymerase-amplified PCR products

Analyzing the Transformants 1)        Culture 10 white or light blue colonies overnight in LB medium containing 50 µg/ml ampicillin or 50 µg/ml kanamycin. 2)        Note:  If you transformed One Shot® Mach1™-T1R competent E. coli