- ￥200000
- Polyplus Transfection
- 409-10
- 2025年07月09日
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上海玉博生物科技有限公司
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文献和实验Procedure for Transfection of Mammalian Cells
IMDM containing 20% fetal bovine serum, 1% glutamine, 1% aa In a six-well or 35 mm tissue culture plate, seed ~2x 105 cells per well in 2 ml IMDM containing 10% FBS and nonessential amino acids. Incubate the cells at 37E C
Transfection of siRNA oligos to peritoneal macrophages
Use DeliverX Transfection from Panomics Modified protocol below Step 1: Make complexes Note: Do not scale up more than 3x Thaw siRNA and DeliverX reagent Dilute siRNA into buffer 1 10 uM siRNA 2.17 ul Buffer
Transient transfection into 293T cells
, so you can detach cells with EDTA alone but brief trypsinization gives single suspension. Do not overtreat with trypsin. Transfection of 293 cells The following protocol is for 10 cm dish (medium: 10ml). If you use 6-well or 12-well plates, total
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