SBI特约代理商|PB514B-2 PB514B-2 PB-

Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells

in this protocol, see Figure 2 . View larger version (11K): [in this window] [in a new window]   Figure 2. The pT2/EF1 -MCS-Hygro expression construct. This expression construct contains a ubiquitous

Standard Protocols for the Construction of scFv Libraries

in a phagemid vector ensures natural diversity in the length of the VH CDR3, a higher number of functional scFvs, and soluble scFv expression without the need for subcloning (3 ). Furthermore, phage-display technology also provides a means by which a selected

表达基因克隆技术与图谱构建策略研究

着在此有一个基因。利用CpG岛稀有酶如SacⅡ、BssHⅢ、Eag Ⅰ、HpaⅡ、NotⅠ识别其位点，切割基因组DNA。CpG岛又称HIF岛，即用HpaⅡ酶将CpG岛周围 的DNA切成许多小片段，这些序列称为HIF序列。用位于CpG附近的序列作探针，从总DNA文库 或cDNA文库中得到转录子，鉴定表达基因。 五、基因表达序列分析(Serial analysis of gene expression,SAGE) 特点：Velcule scu VE 等(1995)率先提出的一种分析基因表达的新策略，其主要