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Preparation of Brain Membrane Fractions

. This consists primarily of synaptic plasma membrane.Dilute with TA to 10% sucrose (by adding 3 vol of TA), and pellet by centrifugation at 48,000 x g for 30 min.Resuspend the pellet in water or TA, estimate the protein concentration, aliquot, and store at –70℃

Detection of FMR1 Trinucleotide Repeat Expansion Mutations Using Southern Blot and PCR Methodologies

technical standards for fragile X analysis to supplement the interpretation of molecular genetic results for patients (12 ).

Differentiating Neural Stem Cells into Neurons and Glial Cells

with a magnetic stirrer).        d. Incubate the cells with fluorescence-labeled secondary antibody (5% serum in D-PBS with Ca2 and Mg2 ) in the dark at 37°C for 30–45 minutes.        e. Wash the cells 3X with D-PBS containing Ca2 and Mg