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文献和实验N2 supplement的配置和N2 Supplement操作手册
as a substitute for the N-1 Bottenstein formulation. N-2 medium appears to be selective for neuronal Cell lines and does not support the growth of nonneuronal cell lines. N2 Supplement作用特点以及protocol 应用 •无血清培养 •血清替代品 •神经细胞 瘤生长
The Cell Environment (including types of culture medium)
also has a cost implication not only in terms of medium formulation but also in downstream processing. A 10% FBS supplement contributes 4.8mg of protein per milliliter of culture fluid, which complicates downstream processing procedures. 10.Guidelines for serum
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
实验试剂 Dulbecco’s Modified Eagle Medium (D-MEM) Fetal Bovine Serum (FBS), ESC-Qualified D-MEM/F-12 (1X) with GlutaMAX™-I KnockOut™ Serum Replacement (KSR) MEM Non-Essential Amino Acids Solution, 10 mM
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