BTX特约代理商|47-0204N AGILEPULSE L

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

instrument with an electrode chamber ( The company manufacture three kinds of electrode chambers: GSS-250, GSS-500 or GSS-1000, which differ in the gap distance. All work well for tetraploid production. The choice is based on personal preference

V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法

ofgrowth medium at a density of 105 cells/mL (i.e., a vial containing 5 × 105 cells will seed one 6-cm dish). One 6-cm dish will produce enough cells for over 20Dunn chamber cover slips. 9. Incubate the cells for a further 5 d without refeeding

ES / MEF cell culture and electroporation of targeting construct

BTX cuvette (2 mm gap) in a 630 A Safety Chamber and attach the electrodes securely to the Electro Cell Manipulator 600. Pipette the cells up and down with a 5 ml pipette, and add 800 ul of cells to a microfuge tube containing the targeting construct