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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
Intracellular Immunofluorescent Staining for Flow Cytometry
(100ng/ml) (2hr)/LPS (100ng/ml)(22hr) 2hr/22hr - Brefeldin A MP6-XT22 , TN3-19 Annotations: mouse PEC=mouse thioglycolate-elicited peritoneal macrophages; ConA=Concanavalin A; Iono=Ionomycin
Intracellular Cytokine Staining Protocol
immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinMP6-XT22TNF-aNEW!mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinTN3-19.12Annotations: mouse PEC=mouse thioglycolate
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