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文献和实验Dynabeads® Co-Immunoprecipitation Kit
Dynabeads® Co-Immunoprecipitation Kit Magnet: e.g. DynaMag™-2 Mixer allowing rotation or tilting of tubes. Antibodies of your choice. 实验步骤 Day 1 1. Weigh out the appropriate
Isolate or Deplete Human CD4+ T Cells - Whole Blood, Buffy Coat, MNC
一、实验原理 Dynabeads are mixed with the cell sample in a tube. The Dynabeads will bind to the target cells during a short incubation, and then the bead-bound cells are separated by a magnet. Positive isolation – discard the supernatant and use
Native Chromatin Preparation and Illumina/Solexa Library Construction
) (see Step 39) The kit includes end-repair enzyme mix, end-repair 10X buffer, dNTP solution, and ATP. Dynabeads Protein A (Invitrogen) Ethanol (70% and 100%) Glycerol Glycogen, 20 mg/mL Klenow fragment exo- (optional; see Step
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