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文献和实验cis- Acting DNA control elements, enhancers and promoters, function to control gene expression by acting as targets for specific DNA-binding proteins (trans-acting factors). trans-acting factors are sequence-specific DNA-binding proteins
Sequence-Specific Primed PCR (PCR-SSP) Typing of HLA Class I and Class II Alleles
between the alleles that share the same cis-trans linkeage sequence casettes. In unrelated donor selection, PCR using sequence-specific primers, a flexible and widely used method known to cause less ambiguious results, may be preferable. However, this technique
Analysis of Allele-Specific Gene Expression
. Recent research into cis-acting regulatory polymorphisms has utilized the analysis of allele-specific gene expression to identify functionally important regulatory haplotypes and specific genetic polymorphisms. Allele-specific effects are typically
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