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文献和实验Ribonuclease A (RNase A), DNase-free
is a ~50 kDa protein from cytosol of mammalian cells, e.g., RiboLock™ Ribonuclease Inhibitor.Other inhibitors: uridine 2’,3’-cyclic vanadate, 5’-diphosphoadenosine 3’-phosphate and 5’-diphosphoadenosine 2’-phosphate (2), SDS, diethyl pyrocarbonate, 4 M
MutSDNA Interactions and DNase Protection Analysis with Surface Plasmon Resonance
Elucidation of the mechanisms of recognition of mispaired DNA by MutS and subsequent repair by the multicomponent mismatch repair machinery presents a fascinating challenge (1 ). ATP binding or hydrolysis are implicated in several steps
treatment. Add Plasmid-Safe buffer, ATP, and the ATP-dependent Plasmid-Safe exonuclease as listed below to treat the ligation reaction andthe negative control to increase the effi ciency of cloning bydegrading unligated DNA fragments in the product mixture
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