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文献和实验Harvesting lymphocytes from Peripheral Blood
Blood Harvest ・ Anesthetize mice with 50 m l of Xylene-Ketamine (2:1) coctail ・ Pin animal to a board (use distal extremities) supine ・ Use 70% alcohol to moisten the abdomen
Human Peripheral Blood Mononuclear Cell Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained from a blood bank). Platelets, red blood cells, and neutrophils are largely depleted
FACS Analysis Using Peripheral Blood Cells
FACS Analysis Using Peripheral Blood Cells Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock
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