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上海玉博生物科技有限公司
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文献和实验完全替代Cy3和Rhodamin|性价比高Dylight549荧光二抗
E032310 ¥200/100μl ¥800/500μl 详情 山羊抗兔IgG二抗, 黄红色DyLight 549荧光标记 -DyLight 549 AffiniPure Goat Anti-Rabbit IgG (H+L) EarthOx E032320 ¥200/100μl ¥800/500μl
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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