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100
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上海玉博生物科技有限公司
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet
Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
. , Chiravuri, M. , van der Meer, P. , Feinberg, A.W. , Shi, X. , Shao, Y. , Wu, S.M. , Parker, K.K. , and Chien, K.R. 2009. Generation of functional ventricular heart muscle from mouse ventricular progenitor cells. Science 326:426‐429
that disrupt GSK-3 signaling (e.g., genetargetedcells such as mouse embryonic fibroblasts [MEFs] lacking GSK-3a or GSK-3b, or RNA interference techniques) when examining the involvement of GSK-3 isoforms in the regulationof specific cellular processes
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