avanti特约代理商|850465P-500mg HSPC

Construction and Characterization of Adenovirus Vectors

  Construction and Characterization of Adenovirus Vectors P. Joel Ross and Robin J. Parks Adapted from Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). CSHL Press, Cold Spring Harbor, NY, USA, 2007

Chromatin IP (CHIP assay)

and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time:      2 x 1 ml CHIP lysis buffer      2 x 1 ml CHIP

In Situ Hybridization Using Digoxigenin Labeled Probes

and Washes: 1) Denature the hybridization probe by boiling for 3 minutes and chill on ice 2) Apply 5 µl of hybridization solution on the slides and distribute the solution over the chromosomes with a 18 x 18 mm coverslip. Try to avoid trapping air