ABI特约代理商|AM1836 MagMAX™-96 Vir

Isolation of Total RNA from Difficult Tissues

techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note

PureLink RNA Mini Kit

application. 3.        RNA Storage Store the purified RNA on ice for immediate use. For long–term storage, keep the purified RNA at –80°C. Perform DNase I treatment after purification (refer to the PureLink™ RNA Mini Kit manual) to assure highly

Isolation of Total RNA from Animal Cells use RNeasy Mini Kit

.   注意事项   1.        All steps performed at room temperature.  2.        RNA can be stored at –80°C for short time. 3.        At step 8, it is needed to prolong the treated time for cell >5 x 106.