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文献和实验. Resuspend the pellets on ice with a Dounce glass homogenizer and type "A" pestle (as in step 10 of preparation of MT protein above) in ~3x their volume of 1x column buffer containing 0.2 mM GTP. Transfer the resuspended MTs to a 31.5 ml
Porcine brain tubulin preparation
实验试剂 100g P-11 cellulose phosphate fibrous cation exchanger (Whatman Inc, Clifton NJ) 6L 0.1M HCl 6L 0.1 M NaOH 2L 0.1 M MgSO4 2L 10x Column Buffer ~10 L 1x column buffer 10 M NaOH 300 ml
Purification of Antiserum or Ascites by Protein A/G Chromatography
: Since sodium azide is toxic, wear gloves and handle the stock solution with care.Ⅳ Clarify the antiserum by centrifugation at 15,000 x g for 5 minutes at 4. This step is performed to sediment aggregates of denatured protein and lipid; it is an important step
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