thermo特约代理商|A-11029 Goat anti-

secondary antibody review -- data from 99 publications

epithelial Caco2/bbe cells Bio-Rad 10       rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG   FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars

Anti-HA Immunocytology

-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)  

（共享）ELISA原理与实验方法

, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS