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文献和实验Procedures for In Vitro Production of Bovine Embryos
)Holding medium (EVM024)Amersham Pharmacia Biotech 1-800-526-3593Percoll (17-0891-01)Atlanta Biologicals 1-800-780-7788 Lisa x207Fetal Calf Serum (S11110)Biowhittaker 1-800-638-8174 sales@biowhittaker.com Another source of TL mediaBomed 608-233-5284
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
in Schneider''s medium from Sigma] 50 ml Fetal Bovine Serum (JRH #12103-78P) - Heat Inactivated (aliquoted in -20) 5 ml 1:100 Penicillin-Streptomycin (Gibco #15070-063) (aliquoted in -20) Sterile filter (0.2 µ) Store at 4°C - do not freeze B. ML-DmBG2
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