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文献和实验ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
on a support to keep it level) After hybridization, remove slide from Hybridization chamber (avoid water dripping onto array). Wash array: Protect slide from light at all times until imaged to prevent bleaching of dyes.
tail will not hybridize to the beads and are readily washed away. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase
Fluorescence In Situ Hybridization
min Wash PBS/0.5 M MgCl2, 5 min, twice Postfix with 1% formaldehyde/PBS-Mg l2, 5 min Wash PBS, 5 min Dehydrate slide in 70%, 80%, 100% EtOH, 2 min each Air Dry 5 min Denature slide at 76ºC in 70% Formamide/2xSSC, 5 min Dehydrate slide
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