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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
1
- 保质期:
3年
- 英文名:
Uracil-DNA Glycosylase,heat-labile
- 库存:
68
- 供应商:
北京伊塔生物科技有限公司
- CAS号:
13321
- 规格:
100U
| YT04054 | 四硼酸钾 | Potassium tetraborate tetrahydrate | 12045-78-2 | 500g | 瓶 |
| YT04058 | 氯亚铂酸钾 | Potassium chloroplatinite | 10025-99-7 | 1g | 瓶 |
| YT04060 | 丁二酮肟 | Dimethylglyoxime | 95-45-4 | 25g | 瓶 |
| YT04070 | 氢氧化钙 | Calcium Hydroxide | 1305-62-0 | 500g | 瓶 |
| YT04072 | 过磷酸钙 一水合物 | Calcium phosphate monobasic monohydrate | 10031-30-8 | 500g | 瓶 |
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文献和实验Restriction Enzyme Digestion of DNA
.3.Incubate at the appropriate temperature (usually 37E C) for 1 to 2 hours.4.Run a small aliquot on a gel to check for digestion.5.If the DNA is to be used for another manipulation, heat inactivate the enzyme (if it is heat labile) at 70 E C for 15 min
Southern Blotting:DNA Transfer
1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (small gels), 7' (large gels
Southern Blotting: DNA Transfer
1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (small gels), 7' (large gels) 2. Denaturation: Wash gel in (0.5 M NaOH, 1.5 M NaCl) for 20' (small) to 30' (large). 3. Neutralization: Wash in (0.5 M Tris pH 7.0, 3.0 M NaCl) for 20
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