氧化型低密度脂蛋白 氧化型低密度脂蛋白 名称:氧化型低密度脂蛋白 氧化型低密度脂蛋白 货号:YT2469 英文名称:氧化型低密度脂蛋白 品牌:伊塔生物 CAS规格:2mg,纯度≥98%分子式:C42H72O14,分子量:801.01,溶解性:DMSO,鉴定方法:液相,性状:白色粉末,熔点:194-197℃,密度:1.33g/cm3,保存条件:2-8℃ 避光,运输料件:2-8℃,保质期:3年,产地:中国,类别:生化试剂,库存:23,用途:仅用于科研实验 Phosphodiesterase I 使用方法:
OXIDIZED LOW DENSITY LIPOPROTEIN, HUMAN
Quantity: 2.0mg(milligrams)Protein/vial
Cat.No:YT2469
Concentration: 2.0mg/ml(protein)
Packaging: Oxidized LDL is membrane filtered and aseptically packaged in a solution containing phosphate-buffered saline at PH7.4 and <1μM EDTA-Na2.
Preparation: Human LDL(which was purified to homogeneity via ultra-centrifugation (1.019-1.063g/cc)) is oxidized using 5μM Cu2SO4(oxidant)in PBS at 37℃ for 20 hours. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0 fold further than the native LDL. This type Oxidized LDL is widely used to investigate lipid metabolism, but not induce cell apoptosis. Apoptosis inducement choose High Oxidized LDL from our company.
TBARS: TBARS is determined colorimetrically by using Malondialdehyde as a standard.
starting LDL 0.10±0.5 nmoles of MDA/mg Protein
Ox-LDL 20.0±2.0 nmoles of MDA/mg Protein
Storage&Stability:
This product is stable for 6 weeks after receipt when handled aseptically and stored at 2-8℃.Never Freese
*Special Note:
After prolonged storage, some precipitate may be orved. This is normal for this product. Clarify out the aggregates by spinning in a microfruge for 2 minutes.
Biological Activity:
Sample lots of Solarbio Oxidized LDL are evaluated for receptor binding to peritoneal macrophages in conjunction with our Dil-Ox-LDL and [I-125]Ox-LDL
l Preparations of Oxidized LDL are fairly unstable;plan your experiments in advance and use fresh material.
l FOR RESEARCH USE ONLY
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