Glucagon is a 3,5 kDa hormone secreted by the A cells of the Islets of Langerhans in the pancreas and is involved in regulation of glucose homeostasis thro µgh enhanced synthesis and mobilization of glucose in the liver, and regulation of glucose-stimulated insulin secretion.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubation at 4°C. Sample volume < 100 μL serum, plasma, or tissue culture media.
Species Reactivity:
Mammals
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
Endocrine Kits
Radioimmunoassays (RIA)
Therapeutic Areas:
Obesity
Metabolic Disorders
Label:
125I-Glucagon
Precision, %:
Inter-assay: 11.7 ± 3.0
Intra-assay: 4.9 ± 1.3
Accuracy:
97 ± 0.8%
Detection Methods:
Radioactive
125I
Gene Symbol:
GCG
GLP1
glucagon
GRPP
GLP2
Performance:
ED80 = 44 ± 4 pg/mL
ED50 = 109 ± 5 pg/mL
ED20 = 264 ± 17 pg/mL
Cross Reactivity:
Glucagon is conserved in almost all mammals. This kit may be used to measure glucagon in these samples (e.g. rat, mouse, etc.)