Mouse Liver Microvascular Endothelial Cells (mLivMVECs) were initially isolated from mouse liver tissues of 3-week old C57/Black6 mice and SV40-GFPmLivMVECs were selected from mLivMVECs infected with SV40 and GFP expressing lentiviruses with puromycin. Endothelial Growth Medium is recommended for the expansion of SV40-GFP-mLivMVECs. And we warranty a minimum of 30 passages of the cells if cultured following the detailed protocol described below.小鼠肝脏微血管内皮细胞(mLivMVECs)首先从3周龄C57/Black6小鼠肝脏组织中分离,SV40- gfpmlivmvecs从感染了SV40和GFP表达的puromycin慢病毒的mLivMVECs中选取。建议使用内皮生长培养基扩增SV40-GFP-mLivMVECs。如果按照以下详细的方法培养,我们保证至少30代细胞。