- ￥1560
- Sino Biological
- 北京
- 90944-C08HL
- 2025年08月12日
- WB
- HEK293 Cells
- Cynomolgus
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A DNA sequence encoding the cynomolgus complement C3 (XP_005587776.1) (Met1-Asn1663) was expressed with a polyhistidine tag at the C-terminus.
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
Cynomolgus
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
90944-C08HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
C3
- 抗体英文名：
Cynomolgus complement C3 CHO Cell Lysate (WB positive control)
- 抗体名：
Cynomolgus complement C3 CHO Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：Cynomolgus
裂解液靶点：C3
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：HEK293 Cell Cynomolgus complement C3 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Functional Assays for Complement Regulators
would result in damage to host tissues, or in extreme cases, consumption and effective depletion of complement components. Intrinsic to the complement cascade are multiple strategies for control, such as the inherent lability of the activation enzymes, the C3
of paraoxonase-1 activity in lysate The bioactivity of paraoxonase-1 present in the cell lysate was demonstrated by its ability to hydrolyse 4 mM phenyl acetate in 20 mM Tris HCl buffer pH 8.0, containing 1 mM CaCl2. As a control 10 mM EDTA was added
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
Positive control DNA with bimodular (B)/monomodular (M), B/B, and trimodular (T)/B RCCX modules (Fig. ) Thermal cycler and appropriate PCR
技术资料暂无技术资料 索取技术资料
