A DNA sequence encoding the dermatophagoides pteronyssinus DERP1 (P08176, with mutation Cys 132 Ala and Asn 150 Glu) (Met1-Leu320) was expressed with a polyhistidine tag at the C-terminus.
Dermatophagoides pteronyssinus DERP1 (C132A, N150E) CHO Cell Lysate (WB positive control)(产品说明)
反应种属:Dermatophagoides pteronyssinus
裂解液靶点:DERP1
裂解液应用:WB
裂解液保存条件:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述:HEK293 Cell Dermatophagoides pteronyssinus DERP1 (C132A, N150E) transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主:HEK293 Cells
裂解液制备方法:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议:1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
裂解液稳定性:Samples are stable for up to twelve months from date of receipt.
裂解液使用说明:Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested.