- ￥1900 - 6200
- Sino Biological
- 北京
- TB100029
- 2025年08月12日
- 免疫沉淀
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
2-8℃
- 克隆性：
其他
- 保质期：
12个月
- 目录编号：
TB100029
- 级别：
免疫学试剂
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 应用范围：
免疫沉淀
- 抗体英文名：
Immunoprecipitation Kit -MYC Tag Immunomagnetic Beads
- 抗体名：
Immunoprecipitation Kit -MYC Tag Immunomagnetic Beads
- 规格：
20 Tests/100 Tests
| 规格： | 20 Tests | 产品价格： | ￥1900.0 |
|---|---|---|---|
| 规格： | 100 Tests | 产品价格： | ￥6200.0 |
| Contents | TB100029-20 | TB100029-100 | Storage |
| Myc Tag Immunomagnetic Beads1 | 1 mL | 5 mL | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | 4 mL | 22 mL | -20℃ for 12 months |
| 5×TBST（pH7.4） | Required but not supplied | ||
| 1×TBST（pH7.4） | Required but not supplied | ||
| ddH2O | Required but not supplied | ||
| Myc Tag Positive Cell Lysis | 300 μg | 300 μg | -20℃ for 12 months |
| Alkaline Elution Buffer | 3 mL | 15 mL | 2-8℃ for 12 months |
| Acidity Elution Buffer | 3 mL | 15 mL | 2-8℃ for 12 months |
| Neutralization Buffer | 2 mL | 8 mL | 2-8℃ for 12 months |
| Myc Synthetic Peptide | Not included (refer related product PP100029) | Not included (refer related product PP100029) | -20℃ for 12 months |
[1] Myc Tag Immunomagnetic Beads contain immunomagnetic beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
The Myc Tag Immunomagnetic Beads, conjugated with Myc tag Antibody(100029-MM07), are used for Immuneprecipitation / IP of Myc-tagged proteins expressed in human, in vitro expression systems. For IP, the Immunomagnetic Beads are added to a sample containing Myc-tagged proteins to form an Immunomagnetic Beads-protein complex. The complex is removed from the solution manually against a Magnetic Separator. The bound Myc-tagged proteins are dissociated from the Immunomagnetic Beads using an Elution Buffer.
IP Experimental results
| Items |
Lane |
||
| Sample (30 μg) (Whole cell lysate) |
A | myc-mFBP4-HA Transfected 293 |
|
| B | HA-mFBP4-myc Transfected 293 |
||
| Beads | SBI anti-Myc tag Immunomagnetic beads-30 μL |
||
| WB detection antibody | Anti-Myc Tag Antibody, Mouse Mab (100029-MM07) at 1 μg/mL |
||
| Gel | 13% SDS-PAGE reducing gel |
||
| Secondary antibody | Dylight 800-labeled antibody to mouse IgG (H+L), at 1:7500 dilution |
||
| Items |
Lane |
||
| Sample (30 μg) (Whole cell lysate) |
A | myc-mFBP4-HA Transfected 293 |
|
| B | HA-mFBP4-myc Transfected 293 |
||
| C | myc-mFBP4-HA Transfected 293 |
||
| D | HA-mFBP4-myc Transfected 293 |
||
| Beads | US brand Myc tag magnetic beads-30 μL |
SBI Anti-Myc tag Immunomagnetic beads -30 μL |
|
| WB detection antibody | Anti-Myc Tag Antibody, Mouse Mab (100029-MM07) at 1 μg/mL |
||
| Gel | 13% SDS-PAGE reducing gel |
||
| Secondary antibody | Dylight 800-labeled antibody to mouse IgG (H+L), at 1:5000 dilution |
||
| Items |
Lane |
|||||
| Sample (30 μg) (Whole cell lysate) |
A | myc-mFBP4-HA Transfected 293 |
||||
| B | HA-mFBP4-myc Transfected 293 |
|||||
| C | myc-mFBP4-HA Transfected 293 |
|||||
| D | HA-mFBP4-myc Transfected 293 |
|||||
| E | HA-mFBP4-myc Transfected 293 |
|||||
| F | HA-mFBP4-myc Transfected 293 |
|||||
| Beads | SBI Anti-Myc tag Immunomagnetic beads-30 μL |
US brand Myc tag magnetic beads-30 μL |
||||
| WB detection antibody | Anti-Myc Tag Antibody, Mouse Mab (100029-MM07) at 1 μg/mL |
|||||
| Gel | 13% SDS-PAGE reducing gel |
|||||
| Secondary antibody | Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution |
|||||
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文献和实验1, Sun, W; et al. RNF180 Suppresses Methylation of ADAMTS9 DNA Promotor by Ubiquitinating DNMT3A Inhibiting Metastasis in Gastric Cancer. Research Square
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