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文献和实验Preparation of Yeast DNA Embedded in Agarose Plugs
are preferred. 5. Aspirate off the spheroplast solution from each well and add 6 ml of LDS solution. Incubate with gentle shaking at 37°C for at least 15 min. Remove and add fresh LDS solution. Incubate with gentle shaking at 37°C
Simultaneous analysis of DNA content and surface
- and PE-conjugated antibodies and the far red DNA binding dyes 7-aminoactinomycin D or LDS-751 . Hoechst 33342 and 33258 or DAPI can be used on dual laser (488 nm/UV) instruments for simultaneous analysis with two or three fluorochrome-conjugated
Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities
aberrations as a biomarker to indicate DNA repair proficiency. The assay was used successfully to demonstrate base- and nucleotide-excision repair deficiency in certain polymorphic DNA repair genes, namely XRCC1 751Gln and XPD 312Asn, respectively. In addition
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