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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
ELISA;Immunoprecipitation
- 宿主:
Goat
- 抗原来源:
0
- 适应物种:
Rabbit
- 库存:
大量
- 是否单克隆:
2
- 规格:
2 mg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Rb | ELISA, IP, IEP | Goat | Unconjugated | Polyclonal Antibody |
- ELISA
- Immunoprecipitation
- Immunoelectrophoresis
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Highly purified Rabbit IgG isolated from pooled normal Rabbit Serum. 详细描述见链接:http://www.millipore.com/catalogue/item/AP132
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文献和实验Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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