IgM constitutes about 10% of serum immunoglobulins. IgM is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.
Specificity:
Based on immunoelectrophoresis, the antibody reacts with the Fc5μ portion of the human IgM heavy chain. Does not react with human IgG or IgA, or with the light chains of human immunoglobulins. No reactivity against non-immunoglobulin serum proteins, but the antibody may cross-react with IgM from other species.
Key Applications:
ELISA
Western Blotting
Application Notes:
EIA: 1:5,000-1:100,000.
Western blots: 1:5,000-1:200,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Human
Immunogen:
Prepared from purified human IgM Fc5μ fragment.
Presentation:
Lyophilized. Buffer = PBS-BSA (15 mg/mL) with 0.01% thimerosal. Reconstitute to 2 mL with sterile distilled water.
Antibody Category:
Secondary & Control Antibodies
Antibody Sub-Category:
Fragment Specific Secondary Antibodies
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.