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Rabbit anti-Goat IgG, Fc , HRP

conjugate; 1.5 mL
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  • 询价
  • AP107P
  • 2025年07月08日
  • ELISA;Western Blotting
  • Rabbit
  • Goat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      ELISA;Western Blotting

    • 宿主：

      Rabbit

    • 抗原来源：

      0

    • 适应物种：

      Goat

    • 库存：

      大量

    • 是否单克隆：

      2

    • 规格：

      1.5 mL

    Species Reactivity Key Applications Host Format Antibody Type
    Gt ELISA, WB Rabbit HRP Polyclonal Antibody
    Description:
    Rabbit anti-Goat IgG, Fc , HRP conjugate
    Specificity:
    Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG but not with the light chains on most goat immunoglobulins. No antibody was detected against goat IgM, or against immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.
    Key Applications:
    • ELISA
    • Western Blotting
    Application Notes:
    ELISA and Western Blotting 1:5,000 - 1:100,000

    Immunohistochemistry 1:500 - 1:5,000

    Optimal working dilutions must be determined by the end user.
    Species Reactivity:
    Goat
    Presentation:
    Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6, with 15mg/mL BSA and 0.01% Thimerosal.

    RECONSTITUTION:

    Reconstitute with sterile distilled water to match the volume indicated on the label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
    Storage Conditions:
    Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

    WARNING:

    Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
    Antibody Category:
    Secondary & Control Antibodies
    Antibody Sub-Category:
    Fragment Specific Secondary Antibodies
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    HRP
    Host:
    Rabbit
    Concentration:
    1.0 mg/mL
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    详细描述见链接：http://www.millipore.com/catalogue/item/AP107P

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    图标文献和实验
    相关实验

    • 【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

      我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06

    • Anti-P-CREB

      which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates

    • Western Blot/Anti-P-CREB

      % nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope).   Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000

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