- ￥312
- Sigma-Aldrich
- 进口
- C8532-100G
- 2025年11月28日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
根据瓶身LOT号查询
- 英文名：
Sodium citrate tribasic dihydrate
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
6132-04-3
- 规格：
100G
属性
等级
Molecular Biology
质量水平
200
方案
≥99%
表单
powder
pH值（酸碱度）
7.5-9 (25 °C, 29.4 g/L)
mp
>300 °C (lit.)
溶解性
water: soluble 100 mg/mL
异质活性
DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected
SMILES字符串
O.O.[Na+].[Na+].[Na+].OC(CC([O-])=O)(CC([O-])=O)C([O-])=O
InChI
1S/C6H8O7.3Na.2H2O/c7-3(8)1-6(13,5(11)12)2-4(9)10;;;;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;;;2*1H2/q;3*+1;;/p-3
InChI key
NLJMYIDDQXHKNR-UHFFFAOYSA-K
一般描述
应用
- 抗体回收，用于绵羊卵巢切片的免疫组化分析
- 用作培养农杆菌 C58C1 菌株的诱导培养基的组分
- 从全血中分离人中性粒细胞
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Suppressing Dazl modulates tumorigenicity and stemness in human glioblastoma cells.
Glioblastoma is devastating cancer with a high frequency of occurrence and poor survival rate and it is urgent to discover novel glioblastoma-specific antigens for the therapy. Cancer-germline genes are known to be related to the formation and progression of several cancer types by promoting tumor transformation. Dazl is one such germline gene and is up-regulated in a few germ cell cancers. In this study, we analyzed the expression of Dazl in human glioblastoma tissues and cells, and investigated its significance in proliferation, migration, invasion and chemoresistance of the glioblastoma cell lines. We evaluated the expression of Dazl in different pathologic grades of glioblastoma tissues by immunohistochemistry. We assessed the expression of Dazl in glioblastoma cells and normal human astrocytes (NHA) cells by western blotting and RT-qPCR. Then we generated Dazl knockout glioblastoma cell lines using the CRISPR/Cas9 gene-editing technology to explore the cellular function of Dazl. We detected the proliferation and germline traits via CCK-8 assays and alkaline phosphatase staining, respectively. Boyden chamber assays were performed to measure glioblastoma cell migration and invasion. Crystal violet staining was used to determine the number of viable cells after the treatment of Doxorubicin and Temozolomide. Finally, we used subcutaneous xenograft studies to measure the growth of tumors in vivo. We found that Dazl was upregulated in glioblastoma tissues and glioblastoma cell lines. Dazl knockdown glioblastoma cells showed decreased cellular proliferation, migration, invasion, and resistance in vitro, and inhibited the initiation of glioblastoma in vivo. The glioblastoma cell lines A172, U251, and LN229 were found to express stem cell markers CD133, Oct4, Nanog, and Sox2. The expression of these markers was downregulated in Dazl-deficient cells. Our results indicated that Dazl contributes to the tumorigenicity of glioblastoma via reducing cell stemness. Therefore, cancer-germline genes might represent a new paradigm of glioblastoma-initiating cells in the treatment of malignant tumors.
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
0.1MNaPO4 pH7.0, 10 mM EDTA (store at 4 C) GUS Accelerator contains a ready to use luminescent accelerator (store at 4C) III.PROCEDURE FOR B-GLUCURONIDASE DETECTION Positive Control: Add 1ul B-glucuronidase (20 pg of B-glucuronidase, Sigma G-7896
至1L,分装后高压灭菌。 28、10%十二烷基硫酸钠（SDS）溶液 【配制方法】在900ml水中溶解100g电泳级SDS，加热至68℃助溶，加入几滴浓盐酸调节溶液的pH值至7.2，加水定容至1L，分装备用。 【注意】SDS的微细晶粒易扩散，因此称量时要戴面罩，称量完毕后要清除残留在称量工作区和天平上的SDS,10%SDS溶液无须灭菌。 29、20×SSC溶液 【配制方法】在800ml水中溶解175.3g NaCl和88.2g柠檬酸钠，加入数滴10mol/l NaOH溶液调节pH
C 271 9.10×103 T 260 7.40
技术资料暂无技术资料 索取技术资料
