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- 保存条件:
常温
- 保质期:
根据瓶身LOT号查询
- 英文名:
Uracil
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
66-22-8
- 规格:
25G
属性
生物来源
synthetic (organic)
质量水平
200
产品线
BioReagent
检测方案
≥99% (HPLC)
形式
powder
技术
cell culture | mammalian: suitable
mp
>300 °C (lit.)
溶解性
1 M NaOH: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow
储存温度
room temp
SMILES字符串
O=C1NC=CC(=O)N1
InChI
1S/C4H4N2O2/c7-3-1-2-5-4(8)6-3/h1-2H,(H2,5,6,7,8)
InChI key
ISAKRJDGNUQOIC-UHFFFAOYSA-N
一般描述
应用
生化/生理作用
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文献和实验Protocol for tracking the inheritance patterns of proteins in live Saccharomyces cerevisiae cells using a photo-convertible fluorescent protein.
Tracking the inheritance patterns of proteins (TrIPP) is a live-cell imaging technique used for tracking maternal protein segregation patterns between mother and daughter cells during asymmetric divisions of budding yeast. We use the photo-convertible fluorescent protein Dendra2 fused to a protein of interest (POI). Irreversible conversion from green to red fluorescence allows for parallel monitoring of old and new proteins for several generations. Single-cell quantitative image analysis of time-lapse microscopy gives synthesis and decay rates, as well as segregation patterns of the POI. For complete details on the use and execution of this protocol, please refer to Auboiron et al. (2021).
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国产 10次 300 蛋白质研究 中分子量蛋白标准(14-97KD) 国产 200ug 60 预染低范围蛋白质分子量标准 自产 20次 280 低分子量蛋白标准(6.5-66KD ) Sigma M3913 20 280 高分子量蛋白标准(36-205KD) Sigma M3788 20 280 宽范围分子量蛋白标准(6.5-205KD) Sigma M4038 20 280 ECL超敏发光液(western blot)
L-谷氨酰胺(100×) 1.0ml 2.BudR(5’-Bromo-2-deoxyuridine, 5’-溴-2-脱氧尿嘧啶)溶解10mg/ml BudR于dH 2 O中,0.2μm滤膜过滤除菌,分装后-20℃储存,BudR要在柔光(弱光)下操作。 3.胸腺嘧啶(Thymidine,Thd) 按250μg/ml溶解于dH 2 O中,过滤后分装储存于-20℃。 4.Colcemid(Sigma) 5.0μg/ml溶于
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