- ￥2346
- Sigma-Aldrich
- 进口
- F6886-10MG
- 2026年04月02日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
根据瓶身LOT号查询
- 英文名：
Forskolin
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
66575-29-9
- 规格：
10MG
属性
产品名称
毛喉素, from Coleus forskohlii, ≥98% (HPLC), powder
biological source
Coleus forskohlii
Quality Level
200
assay
≥98% (HPLC)
form
powder
color
white to off-white
solubility
ethanol: soluble 50 mg/mL
SMILES string
CC(=O)O[C@H]1[C@@H](O)[C@H]2C(C)(C)CC[C@H](O)[C@]2(C)[C@@]3(O)C(=O)C[C@@](C)(O[C@]13C)C=C
InChI
1S/C22H34O7/c1-8-19(5)11-14(25)22(27)20(6)13(24)9-10-18(3,4)16(20)15(26)17(28-12(2)23)21(22,7)29-19/h8,13,15-17,24,26-27H,1,9-11H2,2-7H3/t13-,15-,16-,17-,19-,20-,21+,22-/m0/s1
InChI key
OHCQJHSOBUTRHG-KGGHGJDLSA-N
Gene Information
human ... OPRK1(4986), SLC2A10(81031), TNF(7124)
说明
General description
Application
- 诱导原代肝细胞中的mRNA表达。
- 作为原代人肝细胞中的脱脂药物。
- 作为培养基补充剂诱导脂肪来源干细胞(ASC)的分化。
- 囊性纤维化跨膜传导调节因子(CFTR)基因的激活。
- 促进人诱导多能干细胞(hiPSC)分化为内皮细胞。
- 在患有和不患囊性纤维化个体来源的分化呼吸道上皮细胞中，刺激囊性纤维化跨膜传导调节因子(CFTR)介导的氯离子分泌。
Biochem/physiol Actions
Preparation Note
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文献和实验Generation of highly pure motor neurons from human induced pluripotent stem cells.
Generation of human motor neurons (MNs) overcomes the inaccessibility to patient brain tissues and greatly facilitates the research in MN-related diseases. Here, we describe a protocol for generation of neural progenitor cells (NPCs) from human induced pluripotent stem cells (hiPSCs), followed by preparation of functional MNs. The optimized induction condition with the expression of three transcription factors in a single lentiviral vector significantly improved the yield and purity, making it possible to biochemically identify dysregulated factors in diseased neurons. For complete details on the use and execution of this protocol, please refer to Ding (2021), Ding et al. (2021), and Sepehrimanesh and Ding (2020).
In situ hybridisation to alpha satellite sequences (chromosome specific)
Store in a universal at room temperature 25m l De-ionised formamide (Sigma F7508) 15 m l probe eg. Appligene/Oncor #CP5040-B.5 (biotin labelled) or label your own probe (~10
2O，2.8 ml浓度为30％的Acrylamide/Bis（29:1）(Bio-Rad)，1.75 ml的1.5M Tris-CL，pH 8.8(Sigma-Aldrich Ltd.)，70 μl浓度为10％的SDS(Bio-Rad)，70 μl 10 ％APS(Bio-Rad)，2.8μl TEMED(Bio-Rad) •5％SDS-PAGE浓缩胶（0.75 mm）; 1.7 ml ddH 2 O，0.415 ml 浓度为30％的Acrylamide/Bis（29:1）（Bio-Rad），0.315
Bacterial Protein Extraction (mini-scale)
or western blot: detergent-soluble proteins. 5.For inclusion bodies purification,add (lysozime 6µl of 10mg/ml)to the resuspended pellet to a f.c.of 200µg/ml,vortex 1min.Add 1ml of B-Per 1:10 to the suspension and vortex for another 1min.Collect inclusion
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