- ￥745
- Sigma-Aldrich
- 进口
- 49752-10G
- 2025年07月11日
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- 保存条件：
常温
- 保质期：
根据瓶身LOT号查询
- 英文名：
2-Phospho-L-ascorbic acid trisodium salt
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
66170-10-3
- 规格：
10G
属性
生物来源
synthetic
质量水平
200
检测方案
≥95.0% (HPLC)
形式
powder
技术
cell culture | mammalian: suitable
杂质
≤7.5% water (Karl Fischer)
颜色
white to off-white
pH值（酸碱度）
9.0-9.5 (25 °C, 30 g/L in H2O)
mp
260 °C
痕量阴离子
chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg
痕量阳离子
Al: ≤10 mg/kg
As: ≤2 mg/kg
Ba: ≤5 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤10 mg/kg
Mg: ≤10 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg
InChI
1S/C6H9O9P.3Na/c7-1-2(8)4-3(9)5(6(10)14-4)15-16(11,12)13;;;/h2,4,7-9H,1H2,(H2,11,12,13);;;/q;3*+1/p-3/t2-,4+;;;/m0.../s1
InChI key
YRWWOAFMPXPHEJ-OFBPEYICSA-K
应用
生化/生理作用
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文献和实验A Human 3D Cardiomyocyte Risk Model to Study the Cardiotoxic Influence of X-rays and Other Noxae in Adults.
The heart tissue is a potential target of various noxae contributing to the onset of cardiovascular diseases. However, underlying pathophysiological mechanisms are largely unknown. Human stem cell-derived models are promising, but a major concern is cell immaturity when estimating risks for adults. In this study, 3D aggregates of human embryonic stem cell-derived cardiomyocytes were cultivated for 300 days and characterized regarding degree of maturity, structure, and cell composition. Furthermore, effects of ionizing radiation (X-rays, 0.1-2 Gy) on matured aggregates were investigated, representing one of the noxae that are challenging to assess. Video-based functional analyses were correlated to changes in the proteome after irradiation. Cardiomyocytes reached maximum maturity after 100 days in cultivation, judged by α-actinin lengths, and displayed typical multinucleation and branching. At this time, aggregates contained all major cardiac cell types, proven by the patch-clamp technique. Matured and X-ray-irradiated aggregates revealed a subtle increase in beat rates and a more arrhythmic sequence of cellular depolarisation and repolarisation compared to non-irradiated sham controls. The proteome analysis provides first insights into signaling mechanisms contributing to cardiotoxicity. Here, we propose an in vitro model suitable to screen various noxae to target adult cardiotoxicity by preserving all the benefits of a 3D tissue culture.
科学界一直以来都有这样的传统：如果科学家找不到“小白鼠”，或者觉得让他人冒险很不道德，那么就会自己“献身”科学。自我实验就像一首英雄主义的史诗，不过也散发着一丝丝疯狂的气息。下面展示的这10个疯狂的自我实验，看来也是一个比一个癫狂。 10. 音速急刹车 第二次世界大战后，美国空军需要知道飞行员从超音速飞机中弹后能否逃过一劫，因为由音速到接近零的突然减速往往会导致死亡。这种速度的变化会让飞行员受到超过40G甚至50G的压力。（1G
【求助】最近在做hsv tk,所用前药为粉末状GCV（购自sigma），但不知道怎么溶解
qulinyd 最近在做hsv tk,所用前药为粉末状GCV（购自sigma），但不知道怎么溶解合适，还请各位帮帮忙哦 freecell 你从sigma买来的东西，应该有个datasheet吧： Preparation Instructions Soluble at 10 mg/ml in 0.1 N HCl. Storage/Stability Store desiccated
一般-20度保存半年应该没问题 DMSO是无菌的啊 chenfengyiguo 关于PMA溶解分装的帖子好多，据我所知的包括园里和文献，有以下需要解决的问题： 1：PMA作为一种PKC激动剂，常用于THP-1或者U937细胞的诱导为巨噬细胞。分子量616.8 2：一般是买sigma的，1mg包装，使用浓度有100nm/L~160nm/L(指上述细胞),那么1mg可处理10L细胞(160nm/L),故一般买1mg包装就足够
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